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    <ns1:donorDNA>Phage P1 loxP sites, mouse phosphoglycerate kinase promoter (PGK promoter), E. coli neo, rtTA[E. coli tet repressor (TetR), herpes simplex virus (HSV) VP16 activation domain], mouse developmental pluripotency-associated 3 (PGC7) genomic DNA</ns1:donorDNA>
    <ns11:citesAsAuthority rdf:resource="http://rdf.ncbi.nlm.nih.gov/pubmed/17143267"/>
    <ns1:expectedDelivery xml:lang="en">C (3-6 months)</ns1:expectedDelivery>
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    <ns4:application>Cre/loxP system</ns4:application>
    <ns6:brs_depositor xml:lang="ja">中村　肇伸</ns6:brs_depositor>
    <dc:description xml:lang="ja">Dppa3 (PGC7/Stella) 遺伝子のノックアウトマウス (rtTAノックインマウス) 。Dppa3遺伝子の開始コドンをrtTA cDNAとfloxed PGK-neoで置換。雄のホモは繁殖に異常はないが、雌のホモは不妊となる。</dc:description>
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    <dc:description xml:lang="en">Dppa3 (PGC7/Stella) gene knockout (rtTA knock-in mice).  The start codon of Dppa3 was replaced with rtTA cDNA and floxed PGK-neo cassette.  Dppa3 gene plays a critical role in embryonic development as a maternal facotor.  Homozygote males mice are fully fertile, but homozygous females are infertile.</dc:description>
    <ns1:strainDevelopment xml:lang="en">Developed by Toshinobu Nakamura, Toru Kimura and Toru Nakano, Graduate School of Medicine and Frontier Biosciences, Osaka University in 2003.  D3 ES cells were used to generate the mutant mice.  The mice were crossed to C57BL/6 (C57BL/6 semicongenic).</ns1:strainDevelopment>
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    <ns6:brs_depositorOrganization xml:lang="ja">国立大学法人大阪大学</ns6:brs_depositorOrganization>
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    <ns1:expectedDelivery xml:lang="ja">C（3〜6か月）</ns1:expectedDelivery>
    <ns1:ESCellLine>D3 [129S2/SvPas]</ns1:ESCellLine>
    <dc:description xml:lang="ja">Dppa3 (PGC7/Stella) 遺伝子のノックアウトマウス (rtTAノックインマウス) 。Dppa3遺伝子の開始コドンをrtTA cDNAとfloxed PGK-neoで置換。雄のホモは繁殖に異常はないが、雌のホモは不妊となる。,</dc:description>
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    <dcterms:references rdf:resource="http://identifiers.org/pubmed/17143267"/>
    <ns4:application>Tet system</ns4:application>
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    <dcterms:identifier>RBRC01494</dcterms:identifier>
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    <skos:altLabel xml:lang="ja">PGC7 KO</skos:altLabel>
    <skos:altLabel xml:lang="en">PGC7 KO</skos:altLabel>
    <ns6:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/straintype_14"/>
    <ns4:addinfo>&lt;a href='https://brc.riken.jp/mus/pcr01494'&gt;Genotyping protocol -PCR-&lt;/a&gt;</ns4:addinfo>
    <ns6:brs_preservationStatus rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainStatus_3"/>
    <ns4:addinfo>Necessary documents for ordering:&lt;ol&gt;&lt;li&gt;Approval form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_6.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_d.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Order form (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_4.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_b.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Category I MTA: MTA for distribution with RIKEN BRC (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_5.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_c.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;li&gt;Acceptance of responsibility for living modified organism (&lt;A HREF="https://mus.brc.riken.jp/ja/wp-content/uploads/form/form_7.docx"&gt;Japanese&lt;/A&gt; / &lt;A HREF="https://mus.brc.riken.jp/en/wp-content/uploads/form/form_g.docx"&gt;English&lt;/A&gt;)&lt;/li&gt;&lt;/ol&gt;&lt;A HREF="https://www.fbs.osaka-u.ac.jp/labs/nakano/index.html" target="_blank"&gt;Lab HP (Japanese)&lt;/A&gt;</ns4:addinfo>
    <ns6:brs_depositor xml:lang="en">Toshinobu NAKAMURA</ns6:brs_depositor>
    <ns6:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_8"/>
    <ns6:brs_hasAllele rdf:resource="http://metadb.riken.jp/db/mgi_rdf/MGI:5448272"/>
    <ns4:termsAndConditions xml:lang="ja">条件を付加する。利用者は事前に寄託者の提供承諾書を得る。&lt;br&gt;使用目的を明らかにすること。</ns4:termsAndConditions>
    <ns6:brs_depositorOrganization xml:lang="en">Osaka Univ.</ns6:brs_depositorOrganization>
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    </ns6:brs_hasGenomicFeature>
    <ns1:strainDevelopment xml:lang="ja">大阪大学大学院医学研究科/生命機能研究科・中村肇伸先生、木村　透先生、仲野　徹先生（2003）。  D3 ES細胞を用いて作出。C57BL/6セミコンジェニック。</ns1:strainDevelopment>
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    <rdfs:label>B6;129S2-Dppa3&lt;tm1(rtTA)Nkn&gt;/NknRbrc</rdfs:label>
    <rdfs:label xml:lang="en">B6;129S2-Dppa3&lt;tm1(rtTA)Nkn&gt;/NknRbrc</rdfs:label>
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    <rdfs:seeAlso rdf:resource="http://www2.brc.riken.jp/lab/animal/detail.php?brc_no=RBRC01494"/>
    <ns4:termsAndConditions xml:lang="en">The RECIPIENT of BIOLOGICAL RESOURCE shall obtain a prior written consent on use of it from the DEPOSITOR. The RECIPIENT must inform the DEPOSITOR the research project using the BIOLOGICAL RESOURCE.</ns4:termsAndConditions>
    <ns6:brs_strainTypeLink rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainType_17"/>
    <ns6:brs_strainAvailability rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/StrainAvailability_6"/>
    <ns7:SIO_001279 rdf:parseType="Resource">
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    <ns1:colonyMaintenance xml:lang="ja">Heterozygote x Wild-type [C57BL/6NCrSlc]</ns1:colonyMaintenance>
    <ns1:colonyMaintenance xml:lang="en">Heterozygote x Wild-type [C57BL/6NCrSlc]</ns1:colonyMaintenance>
    <ns1:isRecombinant rdf:datatype="http://www.w3.org/2001/XMLSchema#boolean"
    >true</ns1:isRecombinant>
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    <ns6:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC01494"/>
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  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/Phenotype_1002870">
    <ns6:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC01494"/>
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  <rdf:Description rdf:about="http://metadb.riken.jp/db/rikenbrc_mouse/RecoveryTestResult_477">
    <ns6:brs_indicatesStrain rdf:resource="http://metadb.riken.jp/db/rikenbrc_mouse/RBRC01494"/>
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